Autophagy inhibition enhances VES-induced viability suppression in SGC-7901.

<p>SGC-7901 cells were exposed to VES, CQ, or 3-MA alone or in presence of 10 mM 3-MA or 20 μM CQ for 24 h. (A) MTT assay show that combined action of both VES and CQ and VES and 3-MA significantly suppress cell viability compared with VES treatment alone (*, compared with control group, <i>p</i> < 0.05; #, compared with VES group, <i>p</i> < 0.05). (B) and (C) DNA histogram shows that accumulation of S phase cells induced by VES was enhanced by CQ and 3-MA (*, compared with control group, <i>p</i> < 0.05; #, compared with VES group, <i>p</i> < 0.05).