Small scale SGA demonstrates the <i>taz1Δ</i> query mutation survives the <i>HS</i> method better than the <i>C</i> method.

<p><i>his3Δ</i> and <i>taz1Δ</i> strains were crossed with a subset (n = 308, Worksheet B in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0132240#pone.0132240.s026" target="_blank">S1 Tables</a>; many telomere-related) of gene deletions from the deletion library using the two methods. <b>(A)</b> Images of final selection plates from the <i>HS</i> and <i>C</i> methods are shown. The double mutants were grown at 30°C after 3 days. One plate (of a total of 4) having two independent replicates was processed for each method. The 16 diagonal black rectangular boxes indicate a proportion of double deletion pairs showing growth defects in the four SGA plates. The red rectangular box highlights the <i>sde2Δ</i> location. <b>(B)</b> Correlation scatter plots comparing the <i>his3Δ</i> small scale SGA and the <i>his3Δ</i> large scale SGA using the <i>HS</i> method for 308 genes <b>(C)</b> Same as (B) but comparison made using the <i>C</i> method.