Gal-7 increases the sensitivity of DU-145 cells to apoptosis.

<p>(<b>A</b>) Cells were incubated for 16 h with the indicated concentrations of etoposide and tested for apoptosis by measuring Parp-1 cleavage by western blot. (<b>B</b>) Apoptosis was confirmed by counting the number of cells with a fragmented nucleus visualized by DAPI staining. (<b>C</b>) Analysis of Parp-1 cleavage in DU-145 cells treated for 16 h with the indicated concentration of cisplatin. (<b>D</b>) Cell proliferation of DU-145 cells treated with or without 5 μM cisplatin measured by (³H]-thymidine incorporation. All results represent three independent experiments, including a minimum of two independent DU-145 clones. *P ≤ 0.05, **P ≤ 0.01, and ***P ≤ 0.001.