IL-17A and IFN-γ production from Spleen and MLN cells of intragastrically or sublingually immunized IFN-γ^-/- and wild-type mice.

<p>Groups of IFN-γ^<b>-/-</b> and wild-type (WT) were immunized via the SL or IG route with <i>H</i>. <i>pylori</i> lysate antigens and CT (SL or IG) or left unimmunized (Inf) and then challenged with live <i>H</i>. <i>pylori</i> bacteria. At three weeks post challenge mice were sacrificed and spleen and MLN was taken for flow cytometric analysis. Spleen and MLN was taken from individual mice (n = 4) and single cell single cell suspensions were prepared and stimulated <i>in vitro</i> with PMA and Ionomycin and then stained for further flow cytometric analysis. <b>A</b> Gating strategy showing analysis of IL-17A and IFN-γ secreting cells among live CD3+ T cells. <b>B</b> Bar graph shows frequency mean + SD of IL-17A^<b>+</b> cells in spleen and MLN. Data represent one of two independent experiments.