ARHGEF11 localization and actin (F-actin) cytoskeleton immunofluorescence.

<p>(<b>A</b>) Localization of ARHGEF11 in stably transduced NRK cells [-LVC (lentiviral scramble control) or–shRNA3]. ARHGEF11 is located near the nucleus and throughout the cell under control conditions for both LVC and LVC-shRNA3 cells, Under serum free conditions ARHGEF11 appears to be distributed throughout the cell and toward the cell membrane. (<b>B</b>) Immunofluorescence of F-actin in stably transduced NRK cells under control, serum free and fasudil treatment. Cells cultured under serum free conditions develop stress fibers and demonstrate elongation of cells. NRK-shRNA3 cells demonstrate a significant reduction in stress fibers, similar to pharmacological imbibition of ROCK by fasudil, as cell length/width ratio decreases to baseline with genetic knockdown of <i>Arhgef11</i>. *p<0.05, n = 3 independent samples/20 random images per slide (6–10 cells per view). Error bars are ±SD.