Assessment of fungal burden over time.

<p>In panels A, B and C, the number of colony forming units per larvae are shown. At time points 4h, 24h, 72h, 168h and 700h after infection larvae were sacrificed and homogenized. For each larvae, 250 μl undiluted, 50 μl undiluted and 50 μl 1:10 diluted suspension was plated out on Sabouraud-gentamicin agar. In panel A, the fungal burden of larvae infected with 4 mg/larvae (red), 0.4 mg/larvae (blue) or 0.04 mg/larvae (green) mm55 is shown. In panels B and C, the same is demonstrated for strains mm68 and cn796. At all time-points it appeared that the larvae infected with the highest inoculum of <i>M</i>. <i>mycetomatis</i> had the highest concentration of <i>M</i>. <i>mycetomatis</i> in their haemolymph. For the lowest concentration, the CFU dropped below the detection limit as early as 3 days after infection.