LBH589 treatment inhibited the growth of SK-NEP-1 xenograft tumors in nude mice.

<p>SK-NEP-1 cells were injected subcutaneously into five nude mice in every group. Ten days after injection, mice were treatment with PBS, DMSO, and LBH589 10mg/kg and 20mg/kg dose. (A) To clearly demonstrate that LBH589 causes apoptosis in SK-NEP-1 and G401 cells, we assessed the expression of PARP, caspase 3, caspase 9 and Bcl-2, recognized markers of apoptosis by western blotting. Down regulation of Bcl-2 and cleaved PARP and caspase 9 was observed after 24 hours of treatment with 50nM and 100nM LBH589. (B) SK-NEP-1 xenograft tumors from the treatment experiment. (C) Growth curve of SK-NEP-1 cells treated with LBH589, DMSO and PBS. LBH589 significantly inhibited the growth of SK-NEP-1 xenografts (LBH589 10mg/kg: 1.89 ± 0.77 cm³; LBH589 20mg/kg: 1.14 ± 0.55 cm³) compared with the DMSO group (DMSO: 3.01 ± 2.4 cm³) or PBS group (PBS: 2.78 ± 2.20 cm³, ANOVA P < 0.01). (D) Tumor weight of the treatment experiment. LBH589 treatment decreased the weight of the tumors (LBH589 10mg/kg: 1.12 ± 0.36 g; LBH589 20mg/kg: 0.59 ± 0.11 g) compared to DMSO group (DMSO: 2.04 ± 0.29 g) or PBS group (PBS: 1.98 ± 0.31 g, ANOVA P < 0.01). (E) Body mass curve analysis of nude mice in the treatment experiment. The body mass curve of nude mice treated with LBH589 was almost the same as the control group. At the end of the experiment, body weights of nude mice treated with LBH589 were almost same as the control group (LBH589 10mg/kg: 20.42 ± 2.43g; LBH589 20mg/kg: 20.56 ± 2.34g) compared with the DMSO group (DMSO: 21.44 ± 2.37g) or PBS group cells (PBS: 21.10 ± 1.39g, ANOVA P >0.05).