T helper 17 and 22 cell subsets defined by surface CD4, CCR6 (CD196), CD161 and CCR4 (CD194).

<p>A) Representative flow cytometry dot-plot showing lymphocyte gating strategy. B) Representative dot-plot showing that CCR6 is expressed mostly by CD4^<b>+</b> T lymphocytes. C) and D) Dot-plots showing the differential expression of CD161 and CCR4 in the whole lymphocyte region gated in A (C) or in the CD4^<b>+</b>CCR6^<b>+</b> region gated in B (D). The upper left and right subsets of CD4^<b>+</b>CCR6^<b>+</b>CD161^<b>+</b>, CCR4^<b>-</b> and CCR4^<b>+</b> respectively, were recorded for each patient as different Th17 subsets. E) and F) Dot-plots showing the differential expression of CCR10 and CCR4 in the whole lymphocyte region gated in A (C) or in the CD4^<b>+</b>CCR6^<b>+</b> region gated in B (D). The upper left and right subsets of CD4^<b>+</b>CCR6^<b>+</b>CCR10^<b>+</b>, CCR4^<b>-</b> and CCR4^<b>+</b> respectively, were recorded for each patient as different Th22 subsets.