Correlation of concentrations between LC-MS/MS and quantitative real-time RT-PCR for validation of type XIV collagen.

<p>The x-axis represents m-RNA expression of type XIV collagen as determined by quantitative real-time RT-PCR. The y-axis represents protein concentration of type XIV collagen as determined by LC-MS/MS. In the scatter plot, each data point represents protein concentration and m-RNA expression. (A) The protein concentrations are average concentrations of all peptide transitions (614/711, 614/826, and 614/1013) of peptide ITWDPPSSPVK. (A') The protein concentrations are calculated from the best transition (614/711) for peptide ITWDPPSSPVK. (B) The protein concentrations are calculated from the best transition (708/763) for peptide ASAHAITGPPTELITSEVTAR.