Overexpression of miR-34a-resistant <i>SIRT1</i> or <i>MDM4</i> does not inhibit miR-34a-mediated p53 transcriptional activation.

<p><b>(A-C)</b> Overexpression of miR-34a resistant <i>SIRT1</i> (lacking its 3’UTR, immunoblot (<b>B</b>)) does not reduce the miR-34a-mediated increase in the activity of a luciferase reporter driven by a promoter containing 13 p53 consensus binding sites (pG13-luc) (<b>A</b>) or the miR-34a-mediated increase in mRNA of the p53 transcriptional targets <i>CDKN1A</i>, <i>PUMA</i> and <i>TP53INP1</i>, as measured by qRT-PCR (<b>C</b>). <b>(D-F)</b> Same analysis as in (<b>A-C</b>) performed after co-transfecting an HA-tagged <i>MDM4</i> gene with no 3’UTR and containing synonymous mutations of the miR-34a CDS MREs. All graphs show the mean +/- STDEV of at least three independent experiments (*, p<0.05; **, p<0.01, relative to control miRNA-transfected cells, by 2-tailed Student’s t-test).