ETS1 and SP1 suppression are essential for miR-324-5p-inhibited cell migration, invasion and ECM degradation in HCC.

<p><b>A.</b> The expression levels of ETS1 or SP1 in miR-324-5p-inhibitor transfected HCC cells that were transfected with ETS1 or SP1-siRNA, as measured by western blotting; α-Tubulin served as the loading control. <b>B.</b> Quantification of indicated invading cells in a Matrigel-coated Transwell assay. <b>C.</b> Real-time PCR analysis of MMP2 and MMP9 expression in indicated cells. <i>GAPDH</i> served as control. <b>D.</b> The activity of MMP2 and MMP9 in indicated cells determined by ELISA assay. Each bar represents the mean ± SD of three independent experiments. * <i>P</i> <0.05.