AZD2014 and rapamycin inhibited cell proliferation, colony formation and mTOR signaling in lung cancer cells.

<p>Inhibitory effects of AZD2014 and rapamycin on cell proliferation in A549, PC-9 and SK-MES-1 cells (a, b). (c) Influence of AZD2014 and rapamycin on colony-forming ability of A549 and PC-9 cells, evaluated by clonogenic assay. (d) AZD2014 and rapamycin inhibited mTOR signaling in A549 and SK-MES-1 cells as shown by the decreased phosphorylation of mTORand S6 after treatment for 24h. Cell viability was assessed by MTT, and Colony-forming assay was done as described in Materials and Methods. Columns, mean of three determinations; bars, SD. Results shown are representative of three independent experiments. **, <i>P</i>< 0.01; ***, <i>P</i> < 0.001, control versus AZD2014 or rapamycin-treated cells.