Amino acid residues at VP1-98 and VP1-145 of EV71 variants in clinical samples.

<p>++; Positive by both virus isolation and molecular detection.</p><p>+; Virus isolation negative but positive by molecular detection.</p><p>±; Virus isolation negative but positive by molecular detection with a weak signal.</p><p>-; Negative by both virus isolation and molecular detection.</p><p>“KE” represents deduced amino acid residues at VP1-98<u><b>K</b></u> and VP1-145<u><b>E</b></u>. “KE/EE” indicates mixed nucleotides [K(AAA) and E(GAA)] were identified at VP1-98 on sequencing chromatograms obtained from clinical samples by direct RT-PCR. n.d.; sequences could not be determined.</p><p>*Sequence results were determined from the partial VP1 region obtained by the CODEHOP RT-PCR. Amino acid substitutions from the original inoculated viruses are indicated in bold. For amino acid residues in the VP1 region examined, amino acid substitutions were identified only at VP1-98 and VP1-145, except a substitution from VP1-242K to 242Q in a throat swab sample from monkey #5133 at 10 days postinfection.</p><p>Amino acid residues at VP1-98 and VP1-145 of EV71 variants in clinical samples.