Immunohistological quantitation of infected and of apoptotic liver cells.

<p>(A) Corresponding to the representative IHC images shown in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1005049#ppat.1005049.g008" target="_blank">Fig 8</a>, infected cells were counted in absolute numbers on day 20, differentiated by cell type as indicated, in representative 10-mm² areas of liver tissue sections. Symbols represent cell counts from individual mice, with the median values marked. Open circles, mice from the mock-transduction control group; closed circles, mice from the TCR_NLV-transduction therapy group. (B) Corresponding to the representative IHC images shown in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1005049#ppat.1005049.g010" target="_blank">Fig 10</a> and <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1005049#ppat.1005049.s006" target="_blank">S6 Fig</a>, apoptotic (caspase 3⁺) liver cells were counted on day 20 in absolute numbers, differentiated by infected (IE1⁺) and uninfected (IE1^-) liver cells. Further details as in subfigure (A). The calculation of P values for the significance of the observed trend to a reduction in apoptosis events in the therapy group makes no sense in view of the scientific question of the experiment, namely if xenogeneic T cells exert an immunopathology indicated by enhanced apoptosis, which was obviously not the case. Subfigure (C) points to the fact that on day 20 after infection and cell transfer, T cells (black staining of CD3ε antigen as in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1005049#ppat.1005049.g004" target="_blank">Fig 4</a>) were no longer present in either experimental group. Symbols as in subfigure (A).