Dendritic cells acquire more bacteria in the absence of macrophages.

<p>Macrophages were depleted or not and mice were infected. Twenty-four hours post-primary infection, bladders were analyzed by flow cytometry to determine distribution of UPEC in (A) the MHC II^- and MHC II⁺ compartments, (B) neutrophils and monocytes. (C) Plot depicts the total number of DCs present in the bladder 24 hours post-primary infection. (D-E) Plots depict the percentage distribution of UPEC in the MHC II⁺ compartment by cell population (D) DCs and (E) resident macrophages and monocyte-derived macrophages. Each dot represents one mouse, lines are medians. Experiments were repeated 3 times with 4–7 mice per group in each experiment and results pooled. p-values indicated on graphs, Mann-Whitney test.