SIV_MAC, HIV-2, and SIV_SM transduction of human T cells is less efficient than transduction by HIV-1.

<p>(A) Transduction efficiency of VSV G-pseudotyped two-part vectors for HIV-1_NL4-3GFP (white squares), HIV-2_RODGFP (grey triangles), or SIV_MAC239GFP (black circles) on Jurkat T cells. (B) Chimeric vectors were generated in which <i>gag-pol</i> of SIV_MAC239GFP (white squares) was replaced with <i>gag-pol</i> from SIV_SME543 (grey triangles) or SIV_SM041 (black circles). In each case (A and B), VSV G-pseudotyped vectors were generated by plasmid transfection of 293T cells. Vector stocks were normalized by titer on CRFK cells, and then used to challenge Jurkat T cells. 48 hrs post-challenge, the percentage of GFP-expressing cells was determined by FACS. Data is plotted as percent GFP⁺ (infected) cells (Y axis) versus CRFK infectious units (IU) x 1000 (X axis).