T lymphocytes express two major Dlg1 protein variants due to alternative splicing: Dlg1AB and Dlg1B.

<p>(A) Schematic of the analyzed areas of splicing in <i>dlg1</i>. (B) RT-PCR of cDNA from different murine T cells (CD8 = purified unstimulated primary OT-1 CD8+ T cells; CTL = purified OT-1 CD8+ T cell stimulated <i>ex vivo</i> with CD3/CD28 and expanded in rIL-2; OT-1H = CD8+ OT-1 hybridoma T cell line; BI-141 T hybridoma cell) using primers that flank the i1A/i1B (top) or i2/i3/i4/i5 (middle) splice region. Primers that lie within L27β were also used (bottom). (C) Schematic of four possible <i>dlg1</i> transcripts expressed in T cells. (D) BI-141 T cells infected with viruses encoding different Dlg1 splice variants were analyzed via western blotting for Dlg1 expression; Lck was used as a loading control. (E) Schematic representation of Dlg1 variants targeted for knockdown using miR-based knockdown viruses. (F) BI-141 T cells infected with knockdown viruses targeting L27β, i1A, i1B or i3 were analyzed via western blotting for Dlg1; Lck was used as a loading control. (G) Schematic representation of the two major Dlg1 protein variants expressed in T cells.