Ferritin mutants result in CNS phenotypes, as revealed by ɑ-Elav staining.

<p>(A) Mutant CNS (B-H) appear twisted and irregular (E, G); often, holes are seen within the ventral nerve cord (white arrows). Holes can range in sizes from small, partially (B, C) or completely (H) interrupting the ventral nerve cord, to large (F). There can also be multiple holes (D), as compared to wild type. Embryonic brains are also disrupted (F, opposite white arrow). Embryos are photographed at stages 14–15. (I) Quantification and distribution of CNS defects in different ferritin mutants that present CNS defects; statistical difference compared to control following a Chi squared test, at p<0.0001, and is denoted by an asterisk (n = 32, 10, and 138 embryos, respectively).