Calibration of the Versatile continuous culture device (VCCD) using batch cultures.

<p>(A) Comparison of 560nm transmittance measured by the VCCD and 600nm absorbance measured by a conventional spectrophotometer of an <i>E</i>. <i>coli</i> culture in LB broth. (B) Relationship between relative 560nm transmittance measured by the VCCD and cell density of an <i>E</i>. <i>coli</i> culture grown in LB broth. Red squares are excluded from the correlation determination because they are not part of the exponential growth phase. (C) Relationship between relative 560nm transmittance measured by the VCCD and cell density of <i>S</i>. <i>cerevisae</i> growing in YPAD 2% glucose medium. (D) Relative 560nm transmittance of ATCC 1161 medium through different pH values generally observed during <i>M</i>. <i>florum</i> growth. (E) Cell density of <i>M</i>. <i>florum</i> growing in ATCC 1161 medium through pH decrease. (F) Relationship between relative 560nm transmittance measured by the VCCD and cell density of an <i>M</i>. <i>florum</i> culture grown in ATCC 1161 medium.