Identification of transcription starts sites upstream of <i>nanI</i> and <i>nanJ</i>.

<p><b>(A)</b>. Primer extension results on RNA isolated from <i>C</i>. <i>perfringens</i> strain 13 using a primer specific for <i>nanI</i> mRNA. The cells were grown on PY (-) or PY plus 1 mg/ml Neu5Ac (+). The GATC lanes show the results of sequencing using the same primer used for the primer extension experiments. The arrows labeled P1, P2, and P3 indicate the position of the putative start sites. The location of P1, P2, P3 and conserved 14 bp elements are shown in the <i>nanI</i> promoter sequence. <b>(B)</b>. Primer extension results for <i>nanJ</i> from <i>C</i>. <i>perfringens</i> strain 13. The graph shows peak fluorescence of the 5’- 6 FAM labeled RT-PCR product for <i>nanJ</i>. Numbers below each peak indicate the length of the product and the peaks on the bottom line show the location of size standards used to estimate the length of the transcripts. The cells were grown on PY (-SA) or PY plus 1 mg/ml Neu5Ac (+SA). The location of P1, P2 and a conserved 14 bp element are shown in the <i>nanJ</i> promoter sequence.