Representative immunohistochemical and lectin histochemical staining of the brain stem and box-plots of cerebral and cerebellar white matter and brain stem illustrating differences in the number of GFAP-(A–F), CD3-(G–L) and BS-1-(M–R) positive cells per mm² in p75^NTR-/PRX^-, p75^NTR+/PRX^-, and p75^NTR+/PRX⁺ areas as well as unlesioned control tissue.

<p>GFAP-positive cells (A, B, C, D; brains stem; brown) are significantly higher in number in all lesioned groups of the white matter (E) compared to control tissue. There is no difference in GFAP immunoreactivity in the brain stem compared to controls (F). CD3-positive cells (G, H, I, J; brain stem; brown) are significantly lower in number in p75^NTR+/PRX⁺ and p75^NTR+/PRX^- lesions, when compared to p75^NTR-/PRX^- areas in the cerebral and cerebellar white matter (K) and in the brain stem (L). The number of BS-1-positive microglia/macrophages (M, N, O, P; brain stem; brown) lacks significant variations between the lesioned groups in any anatomical localization (Q, R). Significance was designated as p < 0.05, as detected by Mann-Whitney-U group wise test. All box-plots show median value, lower and upper quartiles, minimum and maximum (excluding outliers). Black circle: extreme value; grey circle: outlier; asterisk: significant differences between respective groups. Scale bars: (A–D) 100 μm; (G–J) and (M–P) 50 μm.