Identification of AR splice variants in cell lines derived from the Pten null prostate cancer model.

<p><b>a)</b> Identification of mAR variants (a, b, c) in derived cell lines visualized using conventional PCR. <b>b)</b> Real time PCR evaluation of AR variants amplified from E8 cell cDNA visualized by gel electrophoresis. <b>c, left)</b> Detection of AR isoforms in human and mouse prostate cancer cell lines and <b>c, right)</b> densitometry analysis of variant and AR-FL protein expression normalized to β-actin (*, p<0.05). <b>d)</b> Schematic structure of mAR variants (a, b, c) and AR full length (AR-FL). Solid-colored boxes represent the common exons of AR-FL and hatched cassettes indicate the cryptic exons. Bold straight lines refer to the transcribed sequences in their mRNAs. The predicted amino acid sequence translated by m4a and m1c is listed (lower diagrams).