Tri-lineage differentiation capacity of cells over 21 days.

<p>Cells were cultured in monolayer for 21 days in the presence of the appropriate pro-differentiation supplements followed by fixation and staining. Osteogenic samples were stained with alizarin red for calcium deposits, scale = 150 μm. Chondrogenic samples were stained with alcian blue for SGAGs, images are from 1 well of a 24 well plate. Chondrogenic medium frequently caused cells to partially or fully separate from the substrate and contract into pellet-like structures. Adipogenic samples are stained with oil red O for lipid droplets, scale = 80 μm. Images of controls cells cultured in standard maintenance media and stained as appropriate are inset for comparison.