L-CCR4^-/- mice have reductions in TNF-α and KC levels and an increased production of IL-10 in the peritoneal cavity and do not develop a systemic inflammatory response.

<p>(A) TNF-α, KC and IL-10 levels were detected in peritoneal exudates at 6 h after surgery by ELISA, as described in the Methods section. *<i>P</i><0.05 between the L-WT and sham-WT groups; and #<i>P</i><0.05 between the L-CCR4^-/- and L-WT groups. (B) Absolute numbers of neutrophils were determined in the lung tissue (left panel) and BAL (right panel) at 6 h after surgery by MPO assay or cell counts, respectively. *<i>P</i><0.05 between the L-WT and NL-WT groups; and #<i>P</i><0.05 between the L-CCR4^-/- and L-WT groups. (C) TNF-α and IL-10 levels were detected at 6 h after surgery in blood from WT and CCR4^-/- mice in the sham and L-groups. *<i>P</i><0.05 between the L-WT and NL-WT groups; and #<i>P</i><0.05 between the L-CCR4^-/- and L-WT groups. (D) TNF-α, KC and IL-10 levels were detected at 6 h after surgery in BAL by ELISA. *<i>P</i><0.05 between the L-WT and sham-WT groups; and #<i>P</i><0,05 between the L-CCR4^-/- and L-WT groups.