Effect of the administration of blocking antibodies against HMGB1 or recombinant human HMGB1 during late disease on pulmonary bacilli loads and cytokines expression.

<p>(A) Bacilli burdens in lungs of mice after 60 days of infection treated during one month with HMGB1 blocking antibodies (black bars) or isotype chiken antibodies as a control (white bars). (B) Pulmonary bacilli loads of BALB/c mice after 60 days of infection with <i>M</i>. <i>tuberculosis</i> strain H37Rv treated during one month with recombinant human HMGB1 (grey bars) or human albumin as a control (white bars) (6 mice per group). (C) Quantitative expression of mRNA of cytokines determined by real-time PCR in lungs from mice after 60 days of infection and treated with HMGB1 blocking antibodies administered each other day during one month and control non-treated animals. (D) Cytokines transcription during late disease in mice treated with recombinant human HMGB1 or non-treated control animals. Asterisks represent statistical significance between control (white bars) and treated groups (grey bars) (p<0.05 student T test).