Dhasingreoside: new flavonoid from the stems and leaves of <i>Gaultheria fragrantissima</i>

<div><p>A new flavonoid, dhasingreoside (<b>1</b>) and seven known compounds, quercetin 3-<i>O</i>-β-d-galacturonopyranoside (<b>2</b>), quercetin 3-<i>O</i>-β-d-galactopyranoside (<b>3</b>), quercetin 3-<i>O</i>-β-d-glucuronopyranoside (<b>4</b>), quercetin 3-<i>O-α-</i>l-rhamnopyranoside (<b>5</b>), (–)-epicatechin (<b>6</b>), salicylic acid (<b>7</b>) and gaultherin (<b>8</b>), have been isolated from the shade-dried stems and leaves of <i>Gaultheria fragrantissima</i>, commonly known as ‘Dhasingre’ in Nepal. The structures were elucidated on the basis of physical, chemical and spectroscopic methods. Among known compounds, five compounds (<b>3</b>–<b>6</b> and <b>8</b>) were isolated for the first time from <i>G. fragrantissima</i>. <i>In vitro</i> antioxidant activity of all the isolated compounds was evaluated by 1,1-diphenyl-2-picrylhydrazyl free radical-scavenging assay. Dhasingreoside (<b>1</b>) and other compounds (<b>2</b>–<b>6</b>) showed significant free radical-scavenging activity.</p>