S100B treatment of macrophages results in increased pro-IL1β expression.

<p>Representative dot plots showing flow cytometric analysis of RAW 264.7 cells using anti-CD11b (PerCP-Cy 5.5) to gate macrophages and anti-pro-IL1β (PE) to detect intracellular staining in untreated cells (<b>A</b>) and in cells treated with 2μM S100B (<b>B</b>). Significant increase in the percentage of CD11b positive cells expressing pro-IL-1β in response to S100B is shown graphically in both RAW 264.7 macrophage cells (*P<0.05) (<b>C</b>) and BMDM (***P<0.001) (<b>D</b>). Error bars indicate SEM n = 4.