Treatment with TM5275 or TM5441 increases intrinsic apoptosis.

<p><b>A</b>. Representative immunoblots for HT1080 and HCT116 cells treated with 25 μM or 50 μM TM5275 or TM5441 as indicated for caspase 8, 9, 3, PARP, and their cleavage products, n = 3. The numbers over the cleaved protein bands indicate the ratio of the cleaved protein pixel density to the corresponding tubulin control band pixel density. <b>B</b>. Representative JC-1 FACS plots of cells treated for 48 hours with 50 μM TM5275 or TM5441. The graphs show the average % (± SD) of JC-1 membrane depolarization in control DMSO-treated and TM inhibitor-treated cells n = 3. * indicates <i>P</i> values compared to DMSO controls < 0.05, ** indicates <i>P</i> < 0.01, *** indicates <i>P</i> < 0.001, and **** indicates <i>P</i> < 0.0001.