Western Blot analysis of effect of apaziquone on apoptotic proteins in oral cancer cells.

<p>Equal amounts of proteins in cell free extracts prepared from AMOS III and SCC4 cells treated with different doses of Apaziquone (250nM, 500nM and 1μM) and untreated as well as vehicle treated control cells were resolved by SDS-PAGE, transferred to PVP membranes, probed with specific antibodies and detected using ECL. Panels show- <b>(A) AMOS III cells and (B) SCC4 cells.</b> A dose dependent increase in cleaved PARP, cleaved Caspase 9 and cleaved Caspase 3 was observed in both oral cancer cell lines tested. β-actin was used as a loading control.