c-Myb enhances transcription from genomic elements with c-Myb footprints.

<p>Luciferase-based reporter assay to study the responsiveness of genomic regions, with one or more c-MYB footprints mapped. (A) Representative Immunoblot of CV1 cells transfected with reporter plasmid and-/+ c-Myb. (B) Map of the pGL4.26 vector used for the luciferase assays. The grey box illustrates the genomic fragment containing a c-Myb footprint or control region. The black box illustrates the minimal promoter. (C) Positive control with three MREs. (D-E) Negative controls, (F-K) genomic loci identified to contain c-Myb footprints. The upper panels show the genomic region in the UCSC browser (hg19) presenting DNase I signals, c-Myb footprints and oligos for selected region. The coordinates for the c-Myb footprint are shown above the illustration. The values are the average from three independent experiments-/+ SEM.