Vaccination regimen based on DNA-gp120-14K priming and MVA-B boosting elicits high frequency of gp120-specific T-cells.

<p><b>(A)</b> Immunization schedule of animals. Balb/c mice were inoculated with 100 μg of respective plasmids or 20 μg of proteins intradermally or with 2×10⁷ PFU of recombinant MVA virus expressing gp120 and Gag-Pol-Nef of HIV clade B virus (MVA-B) via intraperitoneal route. The total Env-specific CD4⁺<b>(B)</b> and CD8⁺ (<b>C)</b> T-cell responses induced by the vaccination during adaptive and memory phases were measured by stimulating the splenocytes with overlapping peptides for gp120, as described in Materials and Methods. Data are representative of two independent experiments. * p < 0.05; ** p < 0.005; *** p < 0.001.