Enhancement of polyfunctional Env-specific CD4⁺ T-cells by HIV-1 gp120-14K.

<p>Env-specific CD4⁺ T-cell responses are based on the secretion of CD107a, IFN-γ, TNF-α and IL-2. Polyfunctionality is defined as the ability of the Env-specific CD4⁺ T-cells to secrete a combination of two or more cytokines. <b>(A)</b> Polyfunctionality of Env-specific CD4⁺ T-cells in the adaptive phase. <b>(B)</b> Polyfunctionality of Env-specific CD4⁺ T-cells in the memory phase. Pie charts represent the distribution of cells secreting different combination of cytokines and numbers below indicate the total magnitude. Data are representative of two independent experiments. Statistical significance between the PBS/MVA control animals and vaccinated animals within the polyfunctional population are represented.* p < 0.05; ** p < 0.005; *** p < 0.001.