Histochemical MAD staining using <i>p</i>-nitro blue tetrazolium.

<p>(A) Relative <i>p</i>-NBT staining intensity was expressed as a percentage of the mean optical density in control muscle biopsies (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0132972#sec006" target="_blank">Methods</a>). Horizontal bars represent the mean value of each group. *: Decreased vs. Normal MAD staining, †: Absent vs. Normal MAD staining, ‡: Absent vs. Decreased MAD staining (<i>P</i> < 0.05, Games-Howell <i>post-hoc</i> test). Representative serial cross sections of lateral vastus biopsies: (B) Normal, (C) Decreased, and (D) Absent MAD staining (original magnification: × 100).