Additional file 1: of Acetate: friend or foe? Efficient production of a sweet protein in Escherichia coli BL21 using acetate as a carbon source

Figure S1. Effect of sodium acetate concentration on biomass and recombinant protein yield. Growth curves (A) and SDS-PAGE (B) of the soluble protein extract after small scale cultures in PY medium containing different sodium acetate concentration: 0.4% (blue curve, lane 1); 1% (red curve, lane 2); 1.5% (yellow curve, lane 3); 2.0% (green curve, lane 4) and 2.5% (dark red curve, lane 5). 3Â Îźg of soluble protein extract were loaded in each lane. It appears that increasing acetate concentration in the medium does not affect the efficiency of protein expression, whereas, without pH or pO2 control, biomass tends to decrease with higher acetate concentrations