Functional analysis of miR-34a and miR-335.

<p>(A) Cell viability assays using GIST-T1 cells transfected with miR-34a or miR-335 mimics or a negative control. Cell viabilities were determined 72 h after transfection. Shown are the means of 8 replications; error bars represent standard deviations; <i>P</i> values were determined using Student’s t test. (B) Wound healing assay using GIST-T1 cells transfected with a miRNA mimic or a negative control. Shown on the right are the means of 4 replications; error bars represent standard deviations; <i>P</i> values were determined using Student’s t test. (C,D) Cell migration (C) and Matrigel invasion (D) assays using GIST-T1 cells transfected with a miRNA mimic or a negative control. Arrowheads indicate migrating or invading cells. Shown on the right are the means of 5 random microscopic fields per membrane; error bars represent standard deviations; <i>P</i> values were determined using Student’s t test.