Prevalence of virulence factor genes in major African and French Clonal Complexes causing meningitis.

<p><u>Legend:</u></p><p>^a Genotypic analysis performed using the following parameters: clonal complexes (CC) defined as MLVA types having a maximum distance of changes at 2 loci and a minimum cluster size of 2 types</p><p>^b PMEN clones listed in <a href="http://www.sph.emory.edu/PMEN/" target="_blank">www.sph.emory.edu/PMEN/</a>.</p><p>^c<i>pspA</i> not typable in 3 African and 3 French isolates, both families present in 1 French isolate, no family 3 identified</p><p>^d<i>nanA</i> and <i>nanB</i> not reported because present in 100% of the strain collection</p><p>^e Presence of a truncated gene with lower PM amplified product in 6 French isolates</p><p>Abbreviations: MLVA = Multi Locus Variable number tandem repeats Analysis, Sp = serotype, CC = Clonal Complex, CSF = cerebrospinal fluid, N = number, PMEN = pneumococcal molecular epidemiological network; <i>ply</i> coding for Pneumolysin, <i>pspA</i> for Pneumococcal surface protein A (all types+ family 1/2), <i>pspC</i> for Pneumococcal surface protein C (all types and group 4), <i>pavA</i> for Pneumococcal adhesion and virulence A, <i>lytA</i> for Autolysin A, <i>phtA</i>, <i>B</i>, <i>D</i>, <i>E</i> for Polyhistidine triad complex A, B, D, E, <i>nanA</i>, <i>B</i>, <i>C</i> for Neuraminidase A, B, C, <i>rrgA</i> for detection of Pilus-1 islet, <i>sipA</i> for detection of Pilus2 islet, <i>pcpA</i> for Pneumococcal choline binding protein A, <i>psrp</i> for Pneumococcal serine-rich protein.</p><p>Prevalence of virulence factor genes in major African and French Clonal Complexes causing meningitis.