Expression of angiogenesis- related markers in MSCs after angiogenic differentiation.

<p><b>(A)</b> Expression of mRNA for Gata-2, vWF, Tie-2 and VE-cadherin genes in MCPIP1- overexpressing MSCs after 5 and 10 days of angiogenic differentiation by real time RT-PCR. Fold change in mRNA concentration in MCPIP1- transduced MSCs was computed when compared with Puro-treated cells (calculated as 1). <b>(B)</b> Representative images of angiogenic marker expression assessed with immunocytochemistry in MCPIP1-overexpressing MSCs and Puro- treated MSCs differentiated into endothelial phenotype <i>in vitro</i>. MCPIP1-overexpressing MSCs and Puro were stained against intranuclear transcription factor Gata-2 (Alexa Fluor 488, green) and VE-cadherin (Alexa Fluor 546, red), whereas nuclei were co-stained with DAPI (blue). Cells were analyzed with Leica DM-IRE fluorescent microscope. Scale bars indicate 50μm. <b>(C)</b> Quantitative analysis of angiogenic differentiation of MCPIP1- overexpressing MSCs and Puro cells after 5 and 10d of culture. Graphs represent percentages of cells expressing the indicated angiogenic marker identified by immunohistochemisty within both MSC groups. All results are presented as means ± SD. Statistically significant differences (P<0.05) are shown when compared with Puro (*). Analysis based on three independent experiments. Puro—empty vector-treated MSCs; MCPIP1- MSCs overexpressing MCPIP1.