Anti-DIII sera recognise viral E protein.

<p><b>(A)</b> Immunofluorescence of Vero cells infected with DENV of each serotype, reacted with the serotype-specific anti-DIII pools of sera (top row), the negative control sera (ctrl., middle row) and mAb 4G2 (bottom row). In each case, non-infected cells (n.i., rightmost column) were also used as controls. Bars represent 50 μm. <b>(B)</b> ELISA on whole infective viral particles. Anti-DIII serotype-specific pools diluted to 100 ng/ml and sera from mock-immunised animals were used onto virus particles captured on plates coated with a human serum reactive against all four serotypes. mAb Dengue 1–11 reactive against DENV1 E (at 1 μg/ml) and a Dengue pan-reactive serum against all four serotypes were used as positive controls.