Myotubes are resistant to oxidant-induced loss of cell viability.

<p>Cell viability of myoblasts and myotubes in response to increasing concentration of oxidative stress was measured by LDH release <b>(A, F)</b>, MTT conversion <b>(B, D, E, G)</b>, and cellular NAD+ content <b>(C)</b>. 10μM PJ34 was used in all experiments to test the effect of PARP1 inhibition. Experiments shown in panels <b>A-D</b> were performed with C2C12, and those in panels <b>E-G,</b> with L6 cells. Data represent mean ± SD. One-way ANOVA was used for statistical analysis and for the determination of significance between individual groups. * shows significant difference, <i>p<</i>0.05, in the cell response to H₂O₂ or GOx relative to controls while # shows significant protective effect of PJ34 in H₂O₂ or GOx treated cells, <i>p<</i>0.05. Data shown in <b>A</b>, <b>B</b>, <b>C</b>, <b>D</b>, <b>E</b>, <b>F</b>, and <b>G</b> were calculated as percentage of control cells (untreated myoblasts or myotubes, in each case set as 100%).