Phosphorylation of T259 regulates the AQP5 diffusion coefficient.

<p>(A-B) MDCK wt cells transiently transfected with AQP5-myc-EGFP wt and T259A were treated with 50 μM forskolin or vehicle (DMSO) for 30 min before labeling with QDs and followed by time-lapse microscopy with 20 ms integration for 500 frames at 11.91 Hz. Crops of image sequences were subjected to kICS analysis to determine average diffusion coefficients. (A) Graph showing the average diffusion coefficient, D, in μm²/s over all crops for cells treated with forskolin and DMSO. (B) Diffusion plots showing time decay, -Dt, in μm² versus time, t, in s averaged over all crops for forskolin and DMSO treated cells. Values represent the mean ± standard deviation. * indicates <i>p</i> < 0.05.