The <i>ca10a and ca10b</i> genes play an important role during embryonic development in zebrafish.

<p><b>Left panel:</b> Developmental images of <i>ca10a</i> and <i>ca10b</i> morphant zebrafish injected with two different sets of antisense MOs (MO1 and MO2) over the period of 0 hpf to 5 dpf. <b>A)</b> The top row images show 0 hpf embryos and lateral views of 1–5 dpf uninjected zebrafish larvae with a normal development of organs,. <b>B)</b> <i>ca10a</i> morphant zebrafish injected with 200 μM translational blocking antisense morpholinos (MO1). <b>C)</b> Images of <i>ca10b</i> morphant zebrafish. <b>D)</b> and <b>E)</b> Images of <i>ca10a</i> and <i>ca10b</i> morphants. The images in first panel of <b>D</b> and <b>E</b> show the 1 dpf larvae with rhodamine fluorescence throughout the larvae (indicating successful MO injection) and second panel (<b>D</b> and <b>E</b>) shows the 5 dpf larvae with abnormal appearance. <b>Right panel:</b> Developmental images of <i>ca10a</i> and <i>ca10b</i> CRISPR mutated fish and gRNA controls. <b>F)</b> The top row images show 0 dpf embryos and lateral views of 1–5 dpf gRNA injected zebrafish larvae (control) with a normal development of organs. <b>G)</b> <i>ca10a</i> mutated zebrafish injected with gRNA and <i>cas9</i> mRNA targeted to exon 3 (target site shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0134263#pone.0134263.g007" target="_blank">Fig 7B</a>). <b>H)</b> <i>ca10b</i> mutated zebrafish injected with gRNA and <i>cas9</i> mRNA targeted to exon 1(target site shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0134263#pone.0134263.g007" target="_blank">Fig 7C</a>).