BPA effects during prostatic organoid maturation.

<p>Immunolocalization of the stem cell markers: (<b>A</b>) TROP2 and (<b>B</b>) CD49f show a dose-dependent increase in stem cell focal aggregates when treated with BPA compared to vehicle. (<b>C</b>) Organoids labeled with epithelial cytodifferentiation markers AR and CK8/18 show normal ductal morphology and epithelial differentiation after BPA treatment. Lumens are delineated by white and whole structures by green dotted lines. Scale bars represent 20 μm and all images are representative of n = 3.