<p>(<b>A</b>) Twenty-eight day time course phase-contrast images of a representative Matrigel cultured organoid forming over time from DE-differentiated H9 cells. While images of M-d1, d-4 and d-8 show the entire organoid as it grew, images of M-d20, d-24 and d-28 represent focal areas of formation and elongation of a single duct with extended culture. The representative duct is composed of a putative layer of columnar epithelium (arrows) with central lumens (dotted green lines), surrounded by mesenchyme (arrowheads). All images were obtained at the same magnification, scale bars represent 50 μm. (<b>B</b>) M-d16 and d-22 phase-contrast images following a representative Matrigel cultured organoid differentiated from H1 hESC. The organoid ex...
Background: Organoids are morphologically heterogeneous three-dimensional cell culture systems and s...
<p>Normal and PKDQ4004X cells were plated at a density of 3000 cells per ml of Matrigel with culture...
This protocol describes a strategy for the generation of 3D prostate organoid cultures from healthy ...
<p>Immunostaining analysis by confocal microscopy of 28–30 day organoids for cytodifferentiation and...
<p>Immunolocalization of the stem cell markers: (<b>A</b>) TROP2 and (<b>B</b>) CD49f show a dose-de...
222 biopsy fragments of human hypertrophic prostate tissue were cultured in WAJC 404 serum-free medi...
(A) Cholangiogenesis by H69 cells in 3D culture. Red dotted lines indicate initial formation of a du...
<p>AP-1 cells were grown within Matrigel, fixed and labeled with actin and DAPI. Actin staining reve...
The prostate gland consists of basal and luminal cells arranged as pseudostratified epithelium. In t...
<p>Results represent day 33 organoids (d42 from hPSC) derived from day 9 CHIR cells. For CHIR+F cell...
The prostate epithelium is comprised predominantly of basal and luminal cells. In vivo lineage traci...
The fibromuscular stroma of the prostate regulates normal epithelial differentiation and contributes...
222 human prostatic biopsies were used to prepare cell cultures by means of a medium--colony formati...
<p>(<b>A</b>) Representative micrographs of ERβ immunohistochemistry of primary and secondary coloni...
Prostatic growth and hormonal effects on the prostate play a basic role in the pathogenesis of abnor...
Background: Organoids are morphologically heterogeneous three-dimensional cell culture systems and s...
<p>Normal and PKDQ4004X cells were plated at a density of 3000 cells per ml of Matrigel with culture...
This protocol describes a strategy for the generation of 3D prostate organoid cultures from healthy ...
<p>Immunostaining analysis by confocal microscopy of 28–30 day organoids for cytodifferentiation and...
<p>Immunolocalization of the stem cell markers: (<b>A</b>) TROP2 and (<b>B</b>) CD49f show a dose-de...
222 biopsy fragments of human hypertrophic prostate tissue were cultured in WAJC 404 serum-free medi...
(A) Cholangiogenesis by H69 cells in 3D culture. Red dotted lines indicate initial formation of a du...
<p>AP-1 cells were grown within Matrigel, fixed and labeled with actin and DAPI. Actin staining reve...
The prostate gland consists of basal and luminal cells arranged as pseudostratified epithelium. In t...
<p>Results represent day 33 organoids (d42 from hPSC) derived from day 9 CHIR cells. For CHIR+F cell...
The prostate epithelium is comprised predominantly of basal and luminal cells. In vivo lineage traci...
The fibromuscular stroma of the prostate regulates normal epithelial differentiation and contributes...
222 human prostatic biopsies were used to prepare cell cultures by means of a medium--colony formati...
<p>(<b>A</b>) Representative micrographs of ERβ immunohistochemistry of primary and secondary coloni...
Prostatic growth and hormonal effects on the prostate play a basic role in the pathogenesis of abnor...
Background: Organoids are morphologically heterogeneous three-dimensional cell culture systems and s...
<p>Normal and PKDQ4004X cells were plated at a density of 3000 cells per ml of Matrigel with culture...
This protocol describes a strategy for the generation of 3D prostate organoid cultures from healthy ...