PrtV secretion in type I secretion mutants of <i>V</i>. <i>cholerae</i> O1 El Tor strain C6706.

<p>(A) PrtV secretion was analyzed using culture supernatants of <i>V</i>. <i>cholerae</i> wild type strain C6706 and its mutant derivatives. Lane 1: C6706; lane 2: Δ<i>tolC</i>; lane 3: Δ<i>hlyD</i>; lane 4: Δ<i>prtV</i>. (B) A Coomassie blue stained gel as a control for sample loading. Lane 1: C6706; lane 2: <i>ΔtolC</i>; lane 3: <i>ΔhlyD</i>; lane 4: <i>ΔprtV</i>. (C) PrtV secretion in type II secretion system mutants of <i>V</i>. <i>cholerae</i> O1 El Tor strains. Lanes 1 and 2: culture supernatants of wild type C6706 and Δ<i>prtV</i>; lanes 3, 4 and 5: culture supernatants of wild type 3083, Δ<i>epsC</i> and Δ<i>epsC</i> strain carrying the cloned <i>epsC</i> allele; lanes 6, 7 and 8: culture supernatants of wild type TRH7000, Δ<i>epsC</i> and Δ<i>epsC</i> strain carrying the cloned <i>epsC</i> allele.