Effect of AMPK inhibitor CC and activators AICAR and MET on ROS and lipid peroxidation (MDA) in frozen-thawed chicken sperm.

<p>Sperm were treated in the presence of an AMPK activator (2mM AICAR (in light gray) or 1mM MET (in diagonals)) or an AMPK inhibitor (5µM CC (indark gray)) or Control (Ctrl, in white); Fresh semen (checkered pattern). <b>(A)</b> Lipid peroxidation (malondialdehyde), <b>(B)</b> Reactive oxygen species (ROS).Values represent means ± SEM from 6 different experiments (4 samples/experiment). Different superscripts indicate significant differences between Ctrl and treatments (AICAR, MET or CC) in frozen-thawed semen (^a,b,c, P<0.01). Asterisks indicate statistically significant differences between fresh and frozen-thawed semen (*, P<0.001).