PMA significantly prolonged recovery from inactivation in β_2a containing channels.

<p><b>(A)</b> HEK293 cells were transfected with Ca_V2.2, α₂δ, and β_2a subunits. Inactivation was produced by a 10s prepulse and recovery tracked as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0134117#pone.0134117.g001" target="_blank">Fig 1F</a>. This was done twice in each cell: in some cells PMA was applied 5-min before the second stimulation protocol (left panel), while other cells were used as time-matched controls (i.e. no PMA was applied; right panel). Solid lines show exponential fits to the mean data (left panel: control A = 0.82, t = 40.4 s; PMA A = 0.65, t = 101 s, comparison of fits F = 24.2 p < 0.0001: right panel control A = 0.74, t = 36.9 s; control repeat A = 0.78, t = 55.8 s, comparison of fits F = 2.28 p = 0.1). <b>(B)</b> The time constant of the exponential fit to each individual cell was calculated and the means plotted. PMA significantly prolonged recovery from inactivation (* p < 0.05, ** p < 0.01, <i>ns</i> = “not significant” using ANOVA followed by Bonferroni’s multiple comparison test).