Influence of the expression system on the antibody response of animals immunised with PfEMP1-VarO DBL1 recombinant constructs.

<p><b>(A and B)</b> Titration curves of individual bleed 2 sera collected from five outbred (OF1-1 to -5) (<b>A</b>) or five inbred (BALB/c-1 to -5) mice immunised with bDBL1 (<b>B</b>) on the immunising antigen (bDBL1). OF1 mice responded poorly. <b>(C)</b> Titration curves of serum from a rabbit immunised with bDBL1 compared to the pool of bleed 4 sera from OF1 mice immunised with bDBL1; antigen used in the ELISA: bDBL1. <b>(D)</b> Titration curve of rabbit IgG purified from the serum shown in (<b>C)</b>; antigen used in the ELISA: bDBL1. <b>(E to H)</b> Cross-checking on the homologous and heterologous expression product: titration curves of individual bleed 4 sera from OF1 mice immunised with bDBL1 on bDBL1 <b>(E)</b> and eDBL1 <b>(G)</b>; titration curves of individual bleed 4 sera from OF1 mice immunised with eDBL1 on eDBL1 <b>(F)</b> and bDBL1 <b>(H)</b>.