Ectopic expression of <i>tinman</i> and <i>pannier</i> results in expansion of enhancer activity.

<p>All embryos are stage 13–14 embryos carrying the -2775/-2432 <i>Mef2-lacZ</i> with the Lame duck consensus site mutated. (Left column) accumulation of MEF2; (center column) accumulation of ß-Gal; (right column) merge of prior two channels. Embryos have ectopic ectodermal expression of the following genes: (A-C)no additional genes expressed, note that activity of the enhancer can be seen in all cells of the heart (arrows), but the somatic mesodermal stain is reduced; (D-F)ectopic <i>pnr</i> expression, stains are similar to (A-C); (G-I) ectopic <i>tin</i> expression, note that activity of the enhancer is expanded in the ectoderm and amnioserosa (arrowheads). Arrow indicates cardiac cells. (J-L) ectopic <i>tin</i> and <i>pnr</i> expression, note that the enhancer is more robustly activated in the ectoderm and amnioserosa when compared to (H), which can been seen more dramatically in the inset, and ß-Gal accumulation co-localizes with the MEF2 expression seen in (J). Arrow indicates to cardiac cells. Scale bar, 100μm.