Effect of zinc on CACO-2 tight junctional proteins as a function of the differentiation state of the CACO-2 cell layer.

<p>3-day, 7-day, and 21-day post-confluent CACO-2 cell layers in Falcon 75 cm² culture flasks were refed with control medium or medium containing 100μM Zinc 48 hrs before harvesting in lysis buffer. Further steps were performed as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0133926#pone.0133926.t001" target="_blank">Table 1</a>. Data represents the percent of the zinc condition relative to the normalized control for each age cell layer. Data shown is expressed as the mean ± standard error for an n = 4 cell layers in all cases. NS indicates non significance. * indicates P < 0.05; ** indicates P < 0.01; *** indicates P < 0.001 (Student’s t test, two-tailed).