TIN2-R282H mutation reduces colocalization between endogenous telomerase RNA and telomeres.

<p>(<b>A</b>) Immunostaining-FISH images of WT.1 cells showing co-localization between telomerase RNA and telomeres. Telomeres were identified by immunostaining using a mix of anti-TRF1 and anti-TRF2 antibodies. Telomerase RNA was labeled by RNA FISH. DNA was stained by DAPI. Telomerase RNA spots (red) co-localizing with telomeres (green) are marked by yellow arrows. (<b>B</b>) Quantification of telomerase RNA and telomere co-localization in HCT116 knock-in clones. Plot shows the average number of hTER-telomere co-localization per cell. 25 images (~8–15 cells per image) were taken randomly for each cell line at PD8. All quantifications were carried out blindly. Each point on the plot represents value obtained from one image. Mean values are indicated in red. *** (p≤0.001) ** (p≤0.01) calculated by two-tailed Student’s t-tests.